Objective　The study aimed to verify that ingestion of dietary supplement containing Kaempferia parviflora extract, mulberry leaf extract, green tea extract, chitosan, gymnemic acid and kidney bean extract would increase energy expenditure and fat oxidation of subjects.
Methods　A randomized, double-blind, placebo-controlled, cross-over study was conducted for 34 participants who ingested the dietary supplement containing 150 mg of Kaempferia parviflora extract（2.25 mg of 3,5,7,3',4'-pentamethoxyflavone）, 200 mg of mulberry leaf extract, 200 mg of green tea extract（14 mg of epigallocatechin gallate）, 100 mg of chitosan, 9.4 mg of gymnemic acid and 4.8 mg of kidney bean extract or placebo. The test foods（dietary supplement or placebo）were ingested to subjects 60 minutes before starting of the exercise. The respiratory metabolism was measured at rest（10 minutes）and followed by low intensity exercise（30 minutes）.
Results　The energy expenditure was not significantly different between dietary supplement and placebo during exercise（mean±SE：3469.50±36.21 cal/min for the dietary supplement intake vs. 3475.37±35.58 cal/min for the placebo intake；P＝0.78）. Whereas, intake of dietary supplement significantly increased the fat oxidation compared to that of placebo during exercise（122.93±9.41 mg/min for the dietary supplement intake vs. 104.78±9.68 mg/min for the placebo intake；P＜0.05）.
Conclusions　These results suggest that the dietary supplement increased fat utilization for energy expenditure under low intensity exercise condition.